es2 human ovarian clear cell carcinoma line  (ATCC)

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: (A) In vitro VM in 2 ovarian cancer cell models. Representative capillary-like structure formation at time 0 hour and time 24 hours from ES2 and SKOV3 ovarian cancer cells. (B) Fractal dimension analysis on SKOV3 and ES2 in vitro VM. Fractal dimension, Df = 2 ± 8.0825e-16 was computed for both models. The box-count plots are shown, where the space-filling (red dashed line) denotes the fractal curve filling a 2D unit box to map the recognized VM structures. The actual box-count (blue line) adjusts the space-filling curve to the measured local scaling exponents computed by the algorithm. Here, n(r) is the number of boxes and r is the box size of the structures in the box-counting algorithm. VM indicates vasculogenic mimicry.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques: In Vitro

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Fractal dimension analysis on ES2 model.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: (A) Sample images of 2D wavelet analysis of ES2 cells. The wavelet analyses on the C and CG VM images (upper panels) are shown as decomposed by the MATLAB wavelet analyzer application (lower panels) from the original images, where the axes correspond to the pixel sizes. The wavelet decomposition images were analyzed with the Haar wavelet basis at level 3 where the axes correspond to the wavelet coefficients (diagonal coefficient not shown). (B) Mean branching clusters assessed by 2D wavelet analysis of ES2 VM. Histogram statistics from 2D wavelet analysis in ES2 model showing the mean percolation clusters observed with all 8 catechins tested ( P < .0001, r 2 = 0.999 when comparing gallated vs ungallated groups). VM, vasculogenic mimicry; C, catechin; CG, catechin gallate; EC, epicatechin; ECG, epicatechin gallate; EGC, epigallocatechin; EGCG, epigallocatechin-3-gallate; GC, gallocatechin; GCG, gallocatechin gallate.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Percolation clustering statistics in ES2 model.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Horizontal wavelet coefficients. Higher coefficients generally imply greater levels of cluster connectivity. The globally largest level 3 horizontal mean coefficients of each catechin-treated group observed in the 2D wavelet analysis of ES2 cells. The coefficients are a global measure of the level of convolution at which the branching clusters identified in can be reconstructed using the Haar wavelet basis. C, catechin; CG, catechin gallate; EC, epicatechin; ECG, epicatechin gallate; EGC, epigallocatechin, EGCG, epigallocatechin-3-gallate; GC, gallocatechin; GCG, gallocatechin gallate.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Cell International

Article Title: Deregulation of angiopoietin-like 4 slows ovarian cancer progression through vascular endothelial growth factor receptor 2 phosphorylation

doi: 10.1186/s12935-021-01865-4

Figure Lengend Snippet: ANGPTL4 expression is highest in A2780 and CAOV3 ovarian cancer cells and tumors. a Western blot and statistical graph of angiopoietin-like 4 (ANGPTL4) expression in human ovarian clear cell carcinoma ES2 cells, human ovarian carcinoma A2780 and NCI/ADR-RES cells, and human ovarian adenocarcinoma COC1, SKOV3, OVCAR3, and CAOV3 cells; n = 3 independent experiments per group. b qRT-PCR analysis was performed to determine the expression pattern of ANGPTL4 mRNA in human ovarian cancer cells; n = 3 independent experiments per group. c Immunofluorescence analysis and statistical graph of ANGPTL4 expression in ovarian cancer cells; n = 3 per group. Scale bar: 50 μm. d Western blot and statistical graph of ANGPTL4 expression in the A2780 and CAOV3 xenograft models; n = 4 independent experiments per group. e qRT-PCR analysis of ANGPTL4 mRNA expression was also carried out in A2780 and CAOV3 tumors; n = 4 independent experiments per group. *** p < 0.001 for the indicated comparisons. The data are presented as the mean ± SEM values

Article Snippet: Human ovarian clear cell carcinoma ES2 and human ovarian adenocarcinoma COC1, SKOV3, OVCAR3, and CAOV3 cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Immunofluorescence

Journal: Molecules

Article Title: DNA Photocleavage in the Near-Infrared Wavelength Range by 2-Quinolinium Dicarbocyanine Dyes

doi: 10.3390/molecules25122926

Figure Lengend Snippet: ( A ) A representative superimposed fluorescence microscopy image reveals intracellular dye localization (red) in ES-2 ovarian clear cell adenocarcinoma cells after incubation with 0.5 μg/mL of dye 4 for 24 h followed by staining nuclei with Hoechst 33342 (blue). Inset: The enlarged area of one cell within the fluorescence microscopy image. ( B ) ES2 cancer cell viability for: Cells, no treatment; Light, cells exposed to a 694-nm laser (~1.3 W/cm 2 ) for 5 min; ( 4 ), cells incubated with dye 4 (0.5 μg/mL = 0.9 μM) for 24 h under dark conditions; and ( 4 ) + Light, cells incubated with dye 4 (0.5 μg/mL) for 24 h and exposed to a 694-nm laser (1.3 W/cm 2 ) for 5 min. * p < 0.05 when compared with non-treated cells. Error bars represent standard deviation.

Article Snippet: ES2 human clear cell ovarian carcinoma cell line was obtained from ATCC (Manassas, VA, USA).

Techniques: Fluorescence, Microscopy, Incubation, Staining, Standard Deviation